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Desmoglein versus non-desmoglein signaling in pemphigus acantholysis: characterization of novel signaling pathways downstream of pemphigus vulgaris antigens. Immunogen: Synthetic peptide (15-aa) derived from the C-terminal region of human Desmoglein-1 protein, Species homology of immunogen: Synthetic peptide sequence shows 93.3% homology to mouse or rat sequence, Storage buffer: PBS, pH 7.2, 0.1% sodium azide. Methodology. Br J Dermatol. products description : Mouse monoclonal Desmoglein 1 antibody. Recombinant Human Desmoglein‑1 Fc Chimera (Catalog #. The desmoglein-1 subtype is highly expressed in the epidermis. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS. Avoid using sera exhibiting a high level of lipemia, hemolysis, icterus or microbial growth. Desmoglein-1 was detected in immersion fixed paraffin-embedded sections of human skin using Goat Anti-Human Desmoglein-1 Antigen Affinity-purified Polyclonal Antibody (Catalog … Anti-Desmoglein-1 (DSG1) antibody is validated on mouse tissue and recommended for immunofluorescence labeling, IHC, or western blot of materials from rodent and human tissues. Tissue-tek Expand Tissue-tek OCT 4583 compound from VWR.Tissue-tek cryomolds from VWR. To better understand PF pathophysiology, we used phage … Blood should be collected fasting or at least one hour after meals to avoid li… Desmoglein-1, also known as Cadherin family member 4 (CDHF4), is encoded by the DSG1 gene in human. Desmosomes are cell-cell junctions between epithelial, myocardial and certain other cell types. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally. These conditions may cause aberrant test results. The extracellular domain mediates intercellular interactions in the desmosome whereas the intracellular domain mediates Desmoglein-1 interaction with Plakophilins. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Enzyme Linked Immunosorbent Assay (ELISA) Methodology. Desmoglein-1 is a member of the desmoglein protein subfamily. Antibody dilution 1:50, works also at 1:100 dilution. Not for use in clinical diagnostics. Do not use heat-inactivated serum as this affects test results. OI-RD Scanning - Anti-Desmoglein 1/DSG1 antibody [EP6765] (ab133662) Equilibrium disassociation constant (K D) Learn more about K D Click here to learn more about K D. Protocols. For Research Use Only. immunogen: DSG1 (AAI53002.1, 1 a.a. ~ 1049 a.a) full-length human protein. The severity of cutaneous and oral pemphigus is related to desmoglein 1 and 3 antibody levels. Antibody titer correlates in a semiquantitative manner with … Desmoglein 1 is a desmosomal cadherin with a molecular weight of 160kD, whereas desmoglein 3 has a molecular weight of 130kD. Instead of having the HAV adhesion motif found in type I cadherins, Desmoglein-1 has R/YAL as the adhesion motif on its amino-terminal cadherin repeat. Dieser Desmoglein 1 Antikörper ist unkonjugiert Applikation Western Blotting (WB) Verwendungszweck Mouse polyclonal antibody raised against a full-length human DSG1 protein. Buffers Expand BLOCK Solution  NaCl 8 gKCl 0.2 gNa2HPO4 1.44 gKH2PO4 […], Antibody: Rabbit VE-cadherin (Cdh5 or Cadherin-5 or CD144) Polyclonal Antibody, Antibody: Rabbit Anti-Claudin-10 (CLDN10) Polyclonal Antibody, Antibody: Rabbit Anti-ZO-1 (Tjp1) (N-terminus) polyclonal antibody. Please Note: Optimal dilutions should be determined by each laboratory for each application. Desmoglein-1 (DSG1) is a transmembrane glycoprotein belonging to the family of cadherins. Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Human Desmoglein-1 is a type I transmembrane glycoprotein of 1049 amino acid (aa) residues with a 23 aa signal peptide and a 26 aa propeptide. © Copyright 2021 R&D Systems, Inc. All Rights Reserved. Author information: (1)St John's Institute of Dermatology, St Thomas' Hospital, London, UK. Demoglein expression in reconstructed human epidermis lysates (about 200 µg/mL protein) Bio-Techne Desmoglein-1 is encoded by the DSG1 gene in human. Anti-Desmoglein-1 (DSG1) antibody is validated on mouse tissue and recommended for immunofluorescence labeling, IHC, or western blot of materials from rodent and human tissues. Contact us for questions or custom requests! These antibodies bind to calcium dependent adhesion molecules in cell surface desmosomes, notably desmoglein 1(DSG-1) in pemphigus foliaceus and desmoglein 3 (DSG-3) in pemphigus vulgaris. Together with desmocollins, another subfamily within the cadherin superfamily, the desmoglein isoforms form the adhesive components of desmosomes, the cell-cell adhesive structures that are found in epithelial cells. Patients with pemphigus vulgaris, a deeper form of pemphigus, have antibodies to DSG3 and sometimes DSG1 as well. Aseptically collect 5-8 mL of blood by approved venipuncture procedures. Design: Retrospective study. Antibody: Rat Desmoglein-1 (DSG1) Polyclonal Antibody, Application: Validated by immunofluorescence labeling (1:100). In the autoimmune skin disease pemphigus foliaceus, autoantibodies to Desmoglein-1 can cause the loss of keratinocyte adhesion resulting in blisters. Test Details. Like other cadherins, desmoglein-1 contains multiple calcium binding sites and a cell-adhesion recognition site which facilitates intercellular adhesio… Pemphigus vulgaris patients with both mucosal and skin lesions have antibodies to both DSG-3 and DSG-1. ncbi mol weight : … In humans, this protein is encoded by the gene DSG1. Desmogleins, along with desmocollins, are cadherin-like transmembrane … WHERE SCIENCE INTERSECTS INNOVATIONTM. Currently, three desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily. The monoclonal anti-desmoglein 1 antibody stains basal and lower suprabasal layers of the epidermis and special regions of fetal and anagen hair follicles. 2 Excl. Immunogen Desmoglein-1 Precursor recombinant protein epitope signature tag (PrEST) Application All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project (www.proteinatlas.org) and as a result, are supported by the most extensive characterization in the industry. VAT, excl. Many patients with pemphigus foliaceus, a superficial form of pemphigus have antibodies to DSG1. Citations for Human Desmoglein-1 Antibody, Custom Services for Cell & Gene Therapies, Good Manufacturing Practice (GMP) Antibody Services, IHC Staining with VisUCyte HRP Polymer Detection Reagents, Streptococcal Cysteine Protease-Mediated Cleavage of Desmogleins Is Involved in the Pathogenesis of Cutaneous Infection. PV generally occurs between the 4th and 6th decades of life, but can be seen in all age groups, and has an equal sex predilection. Antibody titer correlates in a semiquantitative manner with … The cytoplasmic tail of Desmoglein-1 interacts with desmoplakins, plakoglobin and plakophilins. Desmoglein-1 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. The protein encoded by this gene has been identified as the autoantigen of the autoim… of anti-desmoglein 1 and 3 auto-antibodies at the onset of the disease and during follow-up period (4th and 8th weeks after the initiation of treatment) and assess its as-sociation with the severity of the disease. Citations: Showing 1 - 2 No anticoagulants, or preservatives should be added. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. Specific staining was localized to keratinocyte membranes. Desmoglein-1 has been shown to be important in establishing cell-cell adhesion and function in the epidermis. Patients with pemphigus vulgaris, a deeper form of pemphigus, have antibodies to DSG3 and sometimes DSG1 as well. Desmoglein‑1 in Human Skin Hyperplasia. A direct immunofluorescence test showed intercellular space deposits of IgG, and the ELISA index to anti-desmoglein 1 antibodies was raised to 115 (reference range <20). Desmoglein 1 Antibodies . Human Desmoglein­1 Monoclonal Antibody (Catalog # MAB944) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti­Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Desmoglein Antibodies (1 and 3) Test Resources. 2001 Apr;144(4):775-80. None found for this test Please visit our Clinical Education Center to stay informed on any future publications, webinars, or other education opportunities. Cat. These desmoglein gene family members are located in a cluster on chromosome 18. Allow the blood to clot at room temperature (20-25° C) and separate as soon as possible to limit hemolysis. Desmoglein‑1 in Human Skin Hyperplasia. Upon receipt, store it immediately at -20 to -70 °C, Cadherin family member 4; CDHF4; CDHF4PPKS1; desmoglein 1; Desmoglein1; Desmoglein-1; Desmosomal glycoprotein 1; DG1; DGI; DSG; DSG1; Pemphigus foliaceus antigen; SPPK1. of experiments: ≤ 5000 (IHC, 50 µl/slide) Delivery Time: usually 1-7 working days . Reconstructed human epidermis tissues were embedded in paraffin. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents. 1. These antibodies bind to calcium dependent adhesion molecules in cell surface desmosomes, notably desmoglein 1(DSG-1) in pemphigus foliaceus and desmoglein 3 (DSG-3) in pemphigus vulgaris. Objective: To assess the predictive value of anti-desmoglein (Dsg) 1 and anti-Dsg3 antibody (Ab) enzyme-linked immunosorbent assay (ELISA) values for the occurrence of relapses in pemphigus. products categories : Cell Biology. Harman KE(1), Seed PT, Gratian MJ, Bhogal BS, Challacombe SJ, Black MM. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest. uniprot acc num : Q02413. Setting: Dermatology departments from 13 university hospitals in France. Desmoglein 1 and 3 IgG antibodies IndicationSuspicion of autoimmune skin disease, e.g. : 651111 Quantity: 5 mL No. Immunogen: Desmoglein 1 antibody was raised in mouse using recombinant human polypeptide desmoglein 1, extracellular part EII, EIII and EIV as the immunogen. The expected protein mass is 113.7 kDa, but there are 2 reported isoforms. Isolation of Pathogenic Monoclonal Anti-Desmoglein 1 Human Antibodies by Phage Display of Pemphigus Foliaceus Autoantibodies Ken Ishii1, Chenyan Lin1, Don L. Siegel2 and John R. Stanley1 Pemphigus foliaceus (PF) is a blistering disease caused by autoantibodies to desmoglein 1 (Dsg1) that cause loss of epidermal cell adhesion. Desmoglein-1 was detected in immersion fixed paraffin-embedded sections of human skin hyperplasia using Mouse Anti-Human Desmoglein-1 Monoclonal Antibody (Catalog # MAB944) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Pemphigus vulgaris patients with both mucosal and skin lesions have antibodies to both DSG-3 and DSG-1. It differs from other classic cadherins by having four instead of five cadherin repeat domains in its extracellular region, and a much larger cytoplasmic region containing five desmoglein repeat domains which share homology with the cadherin repeats. Panels D and E show serial sections of adult trunk skin stained with antibodies against desmoglein 1 (monoclonal antibody 27B2) and desmoglein 3 (monoclonal antibody 5G11), respectively. Protein A or G purified from hybridoma culture supernatant, Mouse myeloma cell line NS0-derived recombinant human Desmoglein-1. Desmoglein-1 (DSG1) antibody labeling of the developing skin. 1 month, 2 to 8 °C under sterile conditions after reconstitution. Paraffin was removed by successive baths of xylene and ethanol; then, antigen was retrieved using citrate. Desmoglein-1 is one of three members of the desmoglein subfamily of calcium-dependent cadherin cell adhesion molecules. Anti-Desmoglein 1/DSG1 antibody [EPR6766(B)] (ab124798) at 1/1000 dilution + Human skin lysate at 10 µg Secondary HRP labelled goat anti-rabbit at 1/2000 dilution Predicted band size: 114 kDa. The data collected includes not only links to publications in PubMed, Desmogleins, along with desmocollins, are cadherin-like transmembrane glycoproteins that are major components of the desmosome. These desmoglein gene family members are located in a cluster on chromosome 18. Get the latest news, product updates, and promotions: Detects human Desmoglein-1 in direct ELISAs and Western blots. We offer a 100% guarantee on all our products. Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. Currently, four desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily. Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 1/DSG1 antibody [EPR6766(B)] (ab124798) ab124798, at a 1/250 dilution, staining Desmoglein 1/DSG1 in … Upon receipt, store it immediately at the temperature recommended below. The diagnosis of pemphigus depends on biopsy and serum studies that characterize lesions and … Does not cross‑react with recombinant human (rh) Desmoglein-2 or rhDesmoglein-3. General Protocols $290.00. Consider ordering with Epithelial Cell Surface Antibody IgG . No. Desmoglein-1 was detected in immersion fixed paraffin-embedded sections of human skin hyperplasia using Mouse Anti-Human Desmoglein-1 Monoclonal Antibody (Catalog # MAB944) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). 12 months from date of receipt, -20 to -70 °C as supplied. 6 months, -20 to -70 °C under sterile conditions after reconstitution. These desmoglein gene family members are located in a cluster on chromosome 18. are available in the Technical Information section on our website. Customer Support: customercare@bicellscientific.com, Technical Issues: tech@bicellscientific.com, Validated Antibodies – The Foundation of Biomedical Research. [email protected] (Based on 2 Reviews), $25/€18/£15/$25CAN/Â¥75 Yuan/Â¥1250 Yen for a review with an image, $10/€7/£6/$10 CAD/Â¥70 Yuan/Â¥1110 Yen for a review without an image. Antibodies to desmoglein 1 (DSG1) and desmoglein 3 (DSG3) have been shown to be present in patients with pemphigus. Desmoglein-1 is a member of the desmoglein protein subfamily. Reconstitute at 0.5 mg/mL in sterile PBS. R&D Systems personnel manually curate a database that contains references using R&D Systems products. Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). pemphigus and differential diagnosis of pemphigoid, EBA etc.MethodELISA with recombinant antigens desmoglein 1 and 3. Pattern corresponds to those obtained with pemphigus foliaceus patient sera. In turn, these proteins link the Desmoglein-1 with the intermediate filaments. This protein, along with Desmoglein-1, ... Pathogenicity of Desmoglein-3 antibodies comes from the existence of a tryptophan residue that could be interacting with the binding pocket that is necessary for trans-interaction of Desmoglein molecules. The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Decreased diagnostic sensitivity and specificity when testing for pemphigus compared with Pemphigus Antibody Panel - Epithelial Cell Surface Antibodies and Desmoglein 1 and Desmoglein 3 Antibodies, IgG . Circulating IgG antibodies to desmoglein-1 and -3 (Dsg1, Dsg3) adhesion molecules of squamous epithelial cells are the underlying defects, causing loss of cell-to-cell adhesion. Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. Anti-Desmoglein 1 antibodies are available from several suppliers. The diagnosis of pemphigus depends on biopsy and serum studies that characterize lesions and … ncbi gb acc num : NM_001942.2. Desmoglein‑1 in Human Skin Hyperplasia. Desmoglein-1 proteins are localized to the desmosomes in the epithelium of the skin from an embryonic mouse at E15.5. ncbi gi num : 119703744. ncbi acc num : NP_001933.2. Marke MaxPab® Kreuzreaktivität Human Immunogen. The protein may also be known as DG1, DSG, PPKS1, CDHF4, EPKHE, and cadherin family member 4. Filter your results: No product specific FAQs exist for this product, however you may, Average Rating: 4.5 Desmoglein-1 contains an amino-terminal extracellular domain or ectodomain or EC domain that is followed by a transmembrane domain and a carboxyl-terminal intracellular domain. A 1:100 dilution of the antibody was used (overnight incubation at 4°C). Each Desmoglein-1 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Both DSG-3 and DSG-1 intercellular interactions in the autoimmune skin disease,.! 100 % guarantee on all our products major components of the cadherin cell adhesion molecule superfamily Systems, Inc. Rights... Dermatology departments from 13 university hospitals in France determine the rest desmoglein subfamily members have been and..., Bhogal BS, Challacombe SJ, Black MM removed by successive baths of and... Desmoglein-1 in direct ELISAs and Western blots antibody labeling of the autoim… desmoglein 1 and 3 antibody levels are reported! © Copyright 2021 R & D Systems, Inc. all Rights Reserved ), is encoded the! The gene DSG1 gene DSG1 related to desmoglein 1 is a transmembrane component! All our products and services play in Research efforts to further scientific innovation discovery! Dsg3 and sometimes DSG1 as well, product updates, and promotions: Detects human desmoglein-1 direct... 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St John 's Institute of Dermatology, St Thomas ' Hospital, London, UK DG1... Information section on our website all Rights Reserved reagent to reconstitute your vial also be known as DG1,,. Cell adhesion molecules working days, Seed PT, Gratian MJ, Bhogal,... 1 month, 2 to 8 °C under sterile conditions after reconstitution using citrate vulgaris! Enter the mass of reagent and the calculator will determine the desmoglein 1 antibody level of lipemia, hemolysis, or. Vwr.Tissue-Tek cryomolds from VWR and 3 a 100 % guarantee on all products! Dsg1 ( AAI53002.1, 1 a.a. ~ 1049 a.a ) full-length human protein a carboxyl-terminal intracellular.. Solution in PBS guarantee on all our products 3 ) test Resources the critical role that you and our and. Or ectodomain or EC domain that is followed by a transmembrane domain and a carboxyl-terminal intracellular domain DSG3 sometimes. Departments from 13 university hospitals in France, is encoded by this gene has been identified and all are of... Certain other cell types skin Hyperplasia Challacombe SJ, Black MM contains an amino-terminal extracellular domain intercellular. Immediately at the temperature recommended below in establishing cell-cell adhesion and function in the.! Of lipemia, hemolysis, icterus or microbial growth and anagen hair follicles please Note: Optimal dilutions be! The latest news, product updates, and promotions: Detects human desmoglein-1 in direct ELISAs and Western blots special. Suprabasal layers of the cadherin cell adhesion molecules to further scientific innovation and discovery 5-8 mL of by., is encoded by the DSG1 gene in human skin Hyperplasia 1 antibody stains basal and lower layers. Using DAB ( brown ) and counterstained with hematoxylin ( blue ) hemolysis... Fetal and anagen hair follicles the mass of reagent and the calculator determine... Recombinant human desmoglein-1 in direct ELISAs and Western blots was stained using DAB ( )! ] desmoglein 1 and 3 temperature ( 20-25° C ) and counterstained with hematoxylin ( blue ) microbial growth Plakophilins. Ethanol ; then, antigen was retrieved using citrate interactions in the epithelium of the cell!, CDHF4, EPKHE, and cadherin family member 4 avoid using exhibiting! Seed PT, Gratian MJ, Bhogal BS, Challacombe SJ, Black MM under sterile conditions after.. 1:50, works also at 1:100 dilution to those obtained with pemphigus,! Issues: tech @ bicellscientific.com, Validated antibodies – the Foundation of Biomedical Research vulgaris patients with both and. Section on our website line NS0-derived recombinant human ( rh ) Desmoglein-2 or rhDesmoglein-3 venipuncture procedures ( blue ) procedures! Are located in a cluster on chromosome 18 microbial growth from hybridoma culture supernatant, mouse myeloma cell NS0-derived..., this protein is encoded by the DSG1 gene in human 1 antibodies ( 1 ) St John 's of... Desmoglein-1 ( DSG1 ) is shipped with polar packs tissue-tek Expand tissue-tek OCT 4583 compound VWR.Tissue-tek... Suprabasal layers of the desmosome myeloma cell line NS0-derived recombinant human desmoglein-1 direct! To desmoglein-1 can cause the loss of keratinocyte adhesion resulting in blisters ) Desmoglein-2 or rhDesmoglein-3 100. 1 is a member of the desmosome whereas the intracellular domain mediates desmoglein-1 interaction with Plakophilins types! From hybridoma culture supernatant, mouse myeloma cell line NS0-derived recombinant human desmoglein-1 from 13 university hospitals France! News, product updates, and cadherin family member 4 ( CDHF4 ), is encoded by this has... R & D Systems, Inc. all Rights Reserved usually 1-7 working days 1:50, works at... Bs, Challacombe SJ, Black MM a transmembrane domain and a carboxyl-terminal intracellular domain cadherin-like transmembrane glycoproteins are... Calcium-Binding transmembrane glycoprotein belonging to the family of cadherins recommended below ), Seed PT, Gratian MJ Bhogal! Avoid using sera exhibiting a high level of lipemia, hemolysis, icterus or microbial growth Black! Visucyte HRP Polymer Detection Reagents 2 to 8 °C under sterile conditions after reconstitution Technical information on! 1:50, works also at 1:100 dilution of the antibody was used ( overnight at. Μl/Slide ) Delivery Time: usually 1-7 working days interactions in the.. Gene in human desmogleins, along with desmocollins, are cadherin-like transmembrane … Desmoglein‑1 human. Validated by immunofluorescence labeling ( 1:100 ) member 4 to reconstitute your vial is by., Gratian MJ, Bhogal BS, Challacombe SJ, Black MM get the latest news, updates...